The result showed 100 % Sensitivity & 100 % Specificity. Results were compared with those obtained by the source Institutes and the sensitivity & specificity were evaluated. of India, New Delhi was evaluated at Cancer Research Centre-Mumbai.

VIROLOGY LABORATORY, CANCER RESEARCH INSTITUTE-MUMBAI: 250 Coded sera originating from five different national institutes (NICB-Delhi, PGIMER-Chandigarh, CMC-Vellore, NARI-Pune, NICED-Kolkata) as sent by Dept.NIMHANS-BANGALORE- NIMHANS BANGALORE: Evaluation report from National HIV Reference Laboratories of Government of India, claiming Sensitivity and Specificity of HIV 1 & 2 Western Blot to be 100 %.IJMM: Evaluation study by Indian journal of Medical Microbiology claimed 100 % Sensitivity & 100 % Specificity of HIV Western Blot.NICD DELHI: Evaluation Report from National Institute of Communicable Diseases claiming 100 % Sensitivity & 100 % Specificity.If HIV specific antibodies are not present, the band pattern does not meet the required criteria. If antibodies to HIV-2 antigens are present, HIV-2 band is also observed along with some of the other bands. If antibodies to HIV-1 antigens are present in the sera, any two ENVELOPE with one or more of the following bands will be seen: p17, p24, p31, gp41, p51/p55, p66, gp120 & gpl60. After washing the unbound conjugate, substrate (BCIP/NBT) is added which results in the staining of bands. Unbound material is washed off and then the strip is incubated with anti-human IgG conjugated to alkaline phosphatase (AP). Antibodies to HIV- 1 & 2 if present, bind to viral antigens located on the strip. To perform the assay, the strip is incubated with the patient serum/plasma diluted in a buffer.

The membrane is cut and packaged as strips. They are then transferred from SDS-PAGE gel on to nitrocellulose membrane, which is also impregnated with HIV-2 antigen(gp36) and a control band. Low molecular weight components migrate faster and are found at the bottom of the gel, while high molecular weight proteins remain near the top. SDS denatures viral components and yields proteins which migrate in the gel according to their molecular weight to produce various bands. The HIV-1 viral antigens are purified and then separated by SDS gel electrophoresis. The HIV 1&2 Western Blot is manufactured from HIV-1 cell line. They are accurate if the procedure is strictly followed. Convenient pack size: 5 Tests & 25 Tests. The ELISA is a type of screening test for antibodies in the serum.Both ELISA and PCR gave positive signals for the first dilution of all. Individual test run possible, separate disposable trays for each strip provided ELISHA, and western blot in detecting evidence of.

Easy interpretation of results through clear test bands & band monitor scale.Confirmatory test for detection of Antibodies to HIV-1 & indicative for HIV-2 antibodies.It is intended to be used as a more specific & supplemental assay on samples found initially reactive using ELISA and other screening tests. HIV 1& 2 WESTERN BLOT is an in vitro qualitative immunoassay for the detection of anti bodies to HIV-1 & HIV-2 in human serum/plasma. Significance Of Malaria Antigens Over Antibodies.

Western blot vs elisa hiv